Revealing of the microRNA involved regulatory gene networks on terpenoid biosynthesis in Camellia sinensis in different growing time points.

作者:Zhao, Shiqi; Wang, Xuewen; Yan, Xiaomei; Guo, Lingxiao; Mi, Xiaozeng; Xu, Qingshan; Zhu, Junyan; Wu, Ailin; Liu, Linlin; Wei, Chaoling

Journal of agricultural and food chemistry

DOI:10.1021/acs.jafc.8b05345

出版年:2018-Nov-06 (Epub 2018 Nov 06)

文献类型:Journal Article
摘要

Tea, made from leaves of Camellia sinensis, has long been consumed worldwide for its unique taste and aroma. Terpenoids play important roles not only in tea beverage aroma formation, but also in the productivity and quality of tea plantation due to their significant contribution to light harvesting pigments and phytohormones. To date, however, the regulation of terpenoid synthase genes remains unclear. Herein, the analyses of metabolomics, sRNAs, degradome and transcriptomics were performed and integrated for identifying key regulatory miRNA-target circuits on terpenoid biosynthesis in leaf tissues over five different months in which the amount of terpenoids in tea leaves varies greatly. Four classes of miRNA-TF pairs which might play a central role in the regulation of terpenoid biosynthesis were also uncovered. Ultimately, a hypothetical model was proposed that mature miRNAs maintained by light regulator at both the transcriptional and posttranscriptional levels negatively regulate the targets to control terpenoid biosynthesis